Uptake of 24Mg by excised pine roots: A preliminary study |
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Authors: | Peter Högberg Paul Jensén Torgny Näsholm Helene Ohlsson |
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Affiliation: | (1) Department of Forest Ecology, Swedish University of Agricultural Sciences (SUAS), S-901 83 Umeå, Sweden;(2) Department of Horticulture, SUAS, P.O. Box 55, S-230 53 Alnarp, Sweden;(3) Department of Forest Genetics and Plant Physiology, SUAS, S-901 83 Umeå, Sweden |
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Abstract: | Uptake of 24Mg by excised roots of Pinus sylvestris L. during up to 4 h long incubations in 99.9 atom % 24Mg (50 M) was measured by ICP-MS. A rapid initial uptake phase (30 min) was followed by a slower uptake. This was interpreted as a shift from a phase dominated by saturable ion exchange (free space uptake), to a non-saturable phase, during which the rate of uptake was 0.077±0.0.012 mol Mg g–1 (d.wt.) h–1. The metabolic uncoupler DNP (2,4-dinitrophenol) at 50 M decreased the Mg uptake rate by 35% only, but the effect of DNP was significant (p<0.01). Several problems related to a high variability in the experimental material were encountered, and further refinement of this approach in studies of plant Mg uptake is suggested. |
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Keywords: | ICP-MS Mg uptake Pinus sylvestris stable isotopes roots |
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