Characterization of several LINE-1 elements in Microtus kirgisorum |
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| Authors: | Vladimir I Mayorov Igor B Rogozin Linda R Adkison |
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| Institution: | (1) Division of Basic Medical Sciences, Mercer University School of Medicine, 1550 College Street, Macon, Georgia 31207, USA, US;(2) Department of Obstetrics and Gynecology, The Medical Center of Central Georgia, Macon, Georgia 31201, USA, US;(3) Institute of Molecular Evolutionary Genetics, Pennsylvania State University, University Park, Pennsylvania 16802, USA, US |
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| Abstract: | Several LINE-1s have been isolated and characterized from genomic DNA of the vole, Microtus kirgisorum. Blot hybridization revealed specific restriction patterns of L1 elements in vole genomes. Rehybridization of the genomic
blot with a cloned 5′-end fragment revealed two major bands indicating the presence of two different L1 subfamilies. The copy
numbers are estimated for different parts of M. kirgisorum L1 elements. Data also demonstrate that most vole L1 elements are truncated at the 5′-end; however, in contrast to mouse,
the ORF1 copy number is higher in vole. A difference between the substitution rates of the ORF1 5′-region (approximately 330
nucleotides) and the rest of the L1 coding regions is revealed.
Received: 12 January, 1999 / Accepted: 18 March, 1999 |
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