Localization of ubiquinone-8 in the Na+-pumping NADH:quinone oxidoreductase from Vibrio cholerae |
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Authors: | Casutt Marco S Nedielkov Ruslan Wendelspiess Severin Vossler Sara Gerken Uwe Murai Masatoshi Miyoshi Hideto Möller Heiko M Steuber Julia |
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Affiliation: | Department of Neuropathology, University of Freiburg, 79106 Freiburg, Germany. |
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Abstract: | Na(+) is the second major coupling ion at membranes after protons, and many pathogenic bacteria use the sodium-motive force to their advantage. A prominent example is Vibrio cholerae, which relies on the Na(+)-pumping NADH:quinone oxidoreductase (Na(+)-NQR) as the first complex in its respiratory chain. The Na(+)-NQR is a multisubunit, membrane-embedded NADH dehydrogenase that oxidizes NADH and reduces quinone to quinol. Existing models describing redox-driven Na(+) translocation by the Na(+)-NQR are based on the assumption that the pump contains four flavins and one FeS cluster. Here we show that the large, peripheral NqrA subunit of the Na(+)-NQR binds one molecule of ubiquinone-8. Investigations of the dynamic interaction of NqrA with quinones by surface plasmon resonance and saturation transfer difference NMR reveal a high affinity, which is determined by the methoxy groups at the C-2 and C-3 positions of the quinone headgroup. Using photoactivatable quinone derivatives, it is demonstrated that ubiquinone-8 bound to NqrA occupies a functional site. A novel scheme of electron transfer in Na(+)-NQR is proposed that is initiated by NADH oxidation on subunit NqrF and leads to quinol formation on subunit NqrA. |
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Keywords: | Bioenergetics Electron Transfer Flavoproteins Membrane Enzymes Respiratory chain Sodium Transport Ubiquinone Membrane Protein Complex Redox Enzyme Sodium Ion Pump |
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