Axillary multiplication of <Emphasis Type="Italic">Swertia chirayita</Emphasis> (Roxb. Ex Fleming) H. Karst., a critically endangered medicinal herb of temperate Himalayas |
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Authors: | Pooja Joshi Vibha Dhawan |
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Institution: | (1) TERI University, Darbari Seth Block, Habitat Place, Lodhi Road, New Delhi, 110 003, India |
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Abstract: | The present study describes the micropropagation of Swertia chirayita, an endangered medicinal herb of the temperate Himalayas, through axillary multiplication from 4-wk-old seedling-derived
nodal explants. We obtained 4.5-fold multiplication every 4 wk on Murashige and Skoog (MS) medium supplemented with 4 μM benzyl
amino purine (BA) and 1.5 μM 6-(γ,γ-dimethylallylamino)purine (2iP). Rooting was optimized on modified MS medium supplemented
with 1 μM naphthalene acetic acid (NAA) and 500 mg l−1 of activated charcoal (AC). A success rate of 94 % was obtained by in vitro hardening in the growth-room and by ex vitro hardening in greenhouse conditions. The present study can serve as a tool for the mass multiplication of elite genotypes
of this critically endangered species and can also be utilized for multiplying large numbers of quality planting material
for the replantation in their natural habitat. This approach can also help meet the requirements of the growing pharmaceutical
industry by ensuring the regular supply of authentic planting material. |
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Keywords: | Conservation Gentianaceae Micropropagation |
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