New effects and applications of thioflavins |
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Authors: | Boris Lakatoš Barbora Kaliňaková Daniela Hudecová Ľudovít Varečka |
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Institution: | 1.Department of Biochemistry and Microbiology, Faculty of Chemical and Food Technology,Slovak University of Technology,Bratislava,Slovak Republic |
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Abstract: | The thiazol dye Thioflavin T (ThT), which is used to stain amyloid fibrils, was found to have strong inhibitory effects on
both growth and conidiation of the deuteromycete Trichoderma viride at concentrations between 10–100 μg/ml (ca. 30–300 μmol/l). Thioflavin S (ThS), also known to stain amyloid fibrils, had no significant effect at these concentrations.
Both stains yielded a fluorescence response, but their distributions were different. ThT was non-homogenously distributed
throughout the cytoplasm, whereas ThS fluorescence was strongly bound to septal regions. The effect of ThT was studied on
several model microorganisms. It exerted a strong inhibitory effect on Staphylococcus aureus (Gram-positive bacterium) (MIC=10 μmol/l), but the effect on Escherichia coli (Gram-negative bacterium) was one order of magnitude less pronounced. The effect on Candida albicans was also very strong (MIC=50 μmol/l). The dermatophytic fungus Microsporum gypseum and deuteromycete Alternaria alternata were less affected by ThT (MIC=250 μmol/l and >500 μmol/l, respectively). These results show that ThT could be a useful inhibitor
of selected microorganisms, whereas ThS could be a useful agent for monitoring formation and maintenance of intrahyphal septa
without inhibiting the growth of the microorganism. |
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