Tissue stretch induces nuclear remodeling in connective tissue fibroblasts |
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Authors: | Helene M Langevin Kirsten N Storch Robert R Snapp Nicole A Bouffard Gary J Badger Alan K Howe Douglas J Taatjes |
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Institution: | (1) Department of Neurology, University of Vermont College of Medicine, 89 Beaumont Ave, Burlington, VT 05405, USA;(2) Department of Orthopaedics and Rehabilitation, University of Vermont College of Medicine, 89 Beaumont Ave, Burlington, VT 05405, USA;(3) Department of Computer Science, University of Vermont, 33 Colchester Ave, Burlington, VT 05405, USA;(4) Department of Medical Biostatistics, University of Vermont College of Medicine, 105 Carrigan Drive, Burlington, VT 05405, USA;(5) Department of Pharmacology, Vermont Cancer Center, University of Vermont College of Medicine, 89 Beaumont Ave, Burlington, VT 05405, USA;(6) Department of Pathology, University of Vermont College of Medicine, 89 Beaumont Ave, Burlington, VT 05405, USA |
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Abstract: | Studies in cultured cells have shown that nuclear shape is an important factor influencing nuclear function, and that mechanical
forces applied to the cell can directly affect nuclear shape. In a previous study, we demonstrated that stretching of whole
mouse subcutaneous tissue causes dynamic cytoskeletal remodeling with perinuclear redistribution of α-actin in fibroblasts
within the tissue. We have further shown that the nuclei of these fibroblasts have deep invaginations containing α-actin.
In the current study, we hypothesized that tissue stretch would cause nuclear remodeling with a reduced amount of nuclear
invagination, measurable as a change in nuclear concavity. Subcutaneous areolar connective tissue samples were excised from
28 mice and randomized to either tissue stretch or no stretch for 30 min, then examined with histochemistry and confocal microscopy.
In stretched tissue (vs. non-stretched), fibroblast nuclei had a larger cross-sectional area (P < 0.001), smaller thickness (P < 0.03) in the plane of the tissue, and smaller relative concavity (P < 0.005) indicating an increase in nuclear convexity. The stretch-induced loss of invaginations may have important influences
on gene expression, RNA trafficking and/or cell differentiation. |
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