Microspore embryogenesis in anther cultures of two Indian cultivars of Brassica juncea (L.) Czern. |
| |
Authors: | K. K. Sharma Sant S. Bhojwani |
| |
Affiliation: | (1) Department of Botany, University of Delhi, 110007 Delhi, India |
| |
Abstract: | Direct microspore-derived embryo formation in anther cultures of two cultivars of Brassica juncea was obtained. Preliminary culture of anthers at 35°C for 1–5 days prior to maintenance at 25°C stimulated embryogenesis. Embryogenesis was also stimulated by an initial culture at 5°C for 3 days. Analysis of squashed anthers revealed that approximately 10% of the microspores began dividing, but less than 1% developed into macroscopic embryos. All embryos transferred to embryo culture medium survived, but only 30% of these developed directly into normal plantlets. The androgenic plants were haploid (2n=18). |
| |
Keywords: | Brassica juncea mustard androgenesis anther culture microspore embryo |
本文献已被 SpringerLink 等数据库收录! |