Controlled expression of functional miR-122 with a ligand inducible expression system |
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Authors: | Cathy M Shea George Tzertzinis |
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Institution: | (1) RNA Biology Division, New England Biolabs, 240 County Road, Ipswich, MA, USA |
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Abstract: | Background To study the biological function of miRNAs, and to achieve sustained or conditional gene silencing with siRNAs, systems that
allow controlled expression of these small RNAs are desirable. Methods for cell delivery of siRNAs include transient transfection
of synthetic siRNAs and expression of siRNAs in the form of short hairpins using constitutive RNA polymerase III promoters.
Systems employing constitutive RNA polymerase II promoters have been used to express miRNAs. However, for many experimental
systems these methods do not offer sufficient control over expression. |
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Keywords: | |
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