Candida zeylanoides as a new yeast model for lipid metabolism studies: effect of nitrogen sources on fatty acid accumulation |
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Authors: | Priscila Dallé da Rosa Paula Mattanna Diórgenes Carboni Lucio Amorim Neila Richards Patricia Valente |
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Institution: | 1. Programa de Pós-gradua??o em Microbiologia Agrícola e do Meio Ambiente (PPGMAA), Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil 2. Departamento de Tecnologia e Ciência dos Alimentos, UFSM, Porto Alegre, RS, Brazil 3. Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil 4. Department of Microbiology, Immunology and Parasitology, Federal University of Rio Grande do Sul, Av. Sarmento Leite, 500, Office 154, 90050-170, Porto Alegre, RS, Brazil
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Abstract: | Lipid homeostasis is well-known in oleaginous yeasts, but there are few non-oleaginous yeast models apart from Saccharomyces cerevisiae. We are proposing the non-oleaginous yeast Candida zeylanoides QU 33 as model. The aim of this study was to investigate the influence of the carbon/nitrogen ratio and the type of nitrogen source upon oil accumulation by this yeast grown on shake flask cultures. The maximum biomass was obtained in yeast extract (2.39?±?0.19 g/l), followed by peptone (2.24?±?0.05 g/l), while the highest content of microbial oil (0.35?±?0.01 g/l) and the maximum lipid yield (15.63 %) were achieved with peptone. Oleic acid was the predominant cellular fatty acid in all culture media (>32.23 %), followed by linoleic (>15.79 %) and palmitic acids (>13.47 %). The highest lipid yield using glucose and peptone was obtained at the C/N ratio of 200:1. |
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