Uptake and metabolism of GABA in astrocytes cultured from dissociated mouse brain hemispheres |
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Authors: | A Schousboe L Hertz G Svenneby |
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Institution: | (1) Department of Biochemistry A, Panum Institute, 2200 Copenhagen N, Denmark;(2) Department of Anatomy, University of Saskatchewan, S7N 0W0 Saskatoon, Canada;(3) Neurochemical Laboratory, The University of Oslo Psychiatric Clinic, Vinderen, Olso 3, Norway |
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Abstract: | Uptake kinetics and contents of GABA in cultured, normal (i.e. nontransformed) glia cells obtained from the brain hemispheres of newborn mice were measured together with the activity of the GABA transaminase. During three weeks of culturing the activity of the transaminase rose from a low neonatal value toward the level in the adult brain. The uptake kinetics indicated an unsaturable component together with an uptake following Michaelis-Menten kinetics. Both theK
m (40 M) and theV
max (0.350 nmol×min–1×mg–1 cell protein) were reasonably comparable to the corresponding values in brain slices, and theV
max was much higher than that reported for other glial preparations. The GABA content was low (<5 nmol/mg cell protein), which is in agreement with the high activity of the GABA transaminase. |
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Keywords: | |
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