DNA synthesis in Hela cells at low temperature |
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Authors: | T Ooka A Girgis J Daillie |
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Affiliation: | 1. Unité de Virologie, Institut National de la Santé, et de la Recherche Médicale, 69008 Lyon, France;2. Section de Biologie Générale et Appliquée, Université Claude Bernard, 69621 Villeurbanne, France |
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Abstract: | It has been proved that 3H-thymidine is incorporated into DNA of HeLa cells cultured at 4 °C and its labelling distribution in DNA is homogeneous. This incorporation of 3H-thymidine increased with the duration of incubation and only 30% of the cell population was labelled after 12 h. When synchronous cell populations were used, the rate and extent of DNA synthesis at 4 °C was proportional to the relative number of cells in S phase at that temperature. Thus, cellular labelling at 4 °C does not result from a non-specific absorption phenomenon, but indicates a DNA synthesis process. |
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