Molecular breeding of aBrevibacterium flavum L-lysine producer using a cloned aspartokinase gene |
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| Authors: | Jane-Hwa Lu Jao-Ling Chen Chii-Cherng Liao |
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| Institution: | (1) Culture Collection and Research Center, Food Industry Research and Development Institute, P. O. Box 246, Hsinchu, Taiwan |
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| Abstract: | Summary ThelysC gene encoding aspartokinase of a lysine-hyperproducing mutantBrevibacterium flavum CCRC 18271 was cloned by polymerase chain reaction and its expression in CCRC 18271 was studied. Enzyme assays showed thatB. flavum cells harbouring thelysC gene exhibited 4- to 11-fold higher specific activities of aspartokinase as compared to respective hosts. Introduction of thelysC gene intoB. flavum CCRC 18271 resulted in increased lysine production up to 33% in flask fermentations. |
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