The effect of Spo0A and AbrB proteins on expression of the genes of guanyl-specific ribonucleases from Bacillus intermedius and Bacillus pumilus in Bacillus subtilis recombinant strains |
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Authors: | V V Ulyanova V I Vershinina M A Kharitonova and M R Sharipova |
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Institution: | (1) Kazan State University, ul. Kremlevskaya 18, Kazan, 420008, Russia |
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Abstract: | Guanyl-specific ribonucleases from Bacillus intermedius and Bacillus pumilus are actively secreted under phosphate starvation by recombinant strains of Bacillus subtilis with native regulatory systems and by strains defective in some proteins of the Spo0A phosphorylation pathway. The level
of expression of ribonuclease genes has been shown to increase approximately sixfold in recombinant strains with mutation
in the spo0A gene and threefold in the spo0A/abrB mutants, as compared with native strains. These results demonstrate that the Spo0A protein regulates the production of ribonucleases
and thus acts as a repressor, while the AbrB protein is an activator of expression of the genes encoding ribonucleases from
Bacillus intermedius and Bacillus pumilus in Bacillus subtilis cells.
Original Russian Text ? V.V. Ul’yanova, V.I. Vershinina, M.A. Kharitonova, M.R. Sharipova, 2007, published in Mikrobiologiya,
2007, Vol. 76, No. 5, pp. 639–644. |
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Keywords: | bacilli binase Bpu RNase regulation of biosynthesis |
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