Localisation of NORs and counterstain-enhanced fluorescence studies inPerca fluviatilis (Pisces,Percidae) |
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| Authors: | B Mayr P Rab M Kalat |
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| Institution: | (1) Veterinary University, Institute for Animal Breeding and Genetics, A-1030 Vienna, Austria;(2) Veterinary University, Ludwig Boltzmann Institute for Immuno- and Cytogenetic Research, A-1030 Vienna, Austria;(3) Institute of Animal Physiology and Genetics, Department of Genetics, Czechoslovak Academy of Sciences, 277 21 Libechov, Czechoslovakia |
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| Abstract: | The karyotype of the perch (Percafluviatilis L.) was analysed by means of silver-staining, the chromomyc in A/3 Distamycin A/DAPI and Actinomycin D fluorescence technique
in order to locate active NORs and to selectively highlight certain heterochromatic regions. The ribosomal RNA genes are localized
at the secondary constrictions of the short arms of chromosomes no. 16. Additionally, bright chromomycin fluorescence was
observed in the same regions. No DAPI/ Distamycin A bright heterochromatic block was detected in the perch karyotype. |
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