Expression of a collagen‐binding domain fusion protein: Effect of amino acid supplementation,inducer type,and culture conditions |
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| Authors: | McKinzie Fruchtl Joshua Sakon Robert Beitle |
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| Affiliation: | 1. Boston Mountain Biotech LLC, Fayetteville, AR;2. Dept. of Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR;3. Ralph E. Martin Dept. of Chemical Engineering, 3202 Bell Engineering Center, University of Arkansas, Fayetteville, AR |
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| Abstract: | Collagen binding domain fusion proteins are of significant importance because of their potential as therapeutic biomaterials. In this paper, we investigate the production of such therapeutic proteins via fermentation of Escherichia coli on both an undefined medium and a defined medium. Defined media with amino acid supplementation provided higher amounts of therapeutic protein than undefined media with no supplementation. Additionally, utilizing lactose instead of isopropyl‐β‐d ‐thio‐galactoside (IPTG) for induction and extending batch time yielded higher amounts of the model therapeutic. © 2014 American Institute of Chemical Engineers Biotechnol. Prog., 31:503–509, 2015 |
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| Keywords: | collagen binding domain fusions fermentation relative cytoplasmic dilution rate total synthesis/accumulation time |
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