Asymbiotic Seed Germination and in vitro Seedling Rapid Development of Paphiopedilum spicerianum

In this paper, we studied the asymbiotic seed germination and seedling development of Paphiopedilum spicerianum, a wild plant with extremely small populations (PSESP) in China. By analogizing the influences of different media, seed maturity, pretreatments and lights on the seed germination, the results showed that the best germination condition is using the seeds at 270 days after pollination, pretreating with 1% NaOCl for 40 min, on the 1/4MS+10% coconut water medium under 24 hours darkness for 4 weeks. For seedling formation, 30g·L-1 Hyponex No 1 medium with 10mg·L-1 α naphthlene acetic acid, 05g·L-1 activated charcoal and 10% banana homogenate was the most effective. For seedling development, the same medium used for seedling formation with supplemented 10g·L-1 6 benzyladenine was most suitable.