Isolation and characterization of a fatty acid binding protein in adipose tissue of Gallus domesticus. |
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Authors: | G H Sams B M Hargis P S Hargis |
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Institution: | Department of Poultry Science, Texas A & M University System, College Station 77843-2472. |
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Abstract: | 1. Fatty acid binding protein (A-FABP) was isolated from chicken adipose cytosol. 2. Relative mol. wt of chicken A-FABP was determined to be 14,400 from SDS-polyacrylamide electrophoresis; the pI was 5.1; and amino acid composition data indicated structural homology with mammalian heart and adipose FABPs. 3. Polyclonal antisera prepared against A-FABP exhibited monospecificity for chicken A-FABP and no cross-reactivity with chicken liver proteins was observed. 4. Determination of relative ligand binding characteristics indicated A-FABP exhibited greatest binding activity in response to linoleate, followed by oleate, palmityl CoA and palmitate; no binding affinity for cholesterol was detected. |
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