Immunolocalization of cholesterol side chain cleavage enzyme (P450scc) in Mytilus galloprovincialis and its induction by nutritional levels |
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Authors: | Ana Alonso Martínez Yolanda Ruiz Muñoz Fuencisla San Juan Serrano Pilar Molist García |
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Affiliation: | Department of Functional Biology and Health Sciences, University of Vigo, 36310 Vigo, Spain. |
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Abstract: | We have examined the localization of P450scc in different tissues of the mollusc Mytilus galloprovincialis along a gonadal cycle by using a polyclonal antibody against rat cholesterol side-chain cleavage enzyme (cytochrome P450scc). Immunoreactivity specific for P450scc was found only in the cytoplasm of basophilic cells from digestive gland; gonad, gills and kidney appeared to be devoid of P450scc immunoreactivity. SDS-gel electrophoresis and western blot analysis of different subcellular fractions revealed that this protein is mainly located in microsomes, has a molecular weight of 51.4 kDa and is recognized by the antibody against rat P450scc. These results suggest that the digestive gland of M. galloprovincialis could be considered a steroidogenic tissue where the first step in biosynthesis of steroid hormones occurs. Moreover, seasonal slot blot analysis of post-mitochondrial fractions showed a P450scc induction by available nutrients and phytoplankton blooms. |
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Keywords: | P450scc Digestive gland Basophilic cells Immunohistochemistry Enzyme induction |
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