Smooth muscle myosin light chain kinase: rapid purification by anion exchange high-performance liquid chromatography |
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| Authors: | L Dalla Libera P Cavallini M Fasolo P Cavanni E Ratti G Gaviraghi |
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| Institution: | National Research Council Unit for Muscle Biology and Physiopathology, University of Padova, Italy. |
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| Abstract: | A rapid procedure for the purification of myosin light chain kinase present in chicken gizzard smooth muscle using anion exchange high-performance liquid chromatography is described. The procedure allows preparation of microgram amounts of the protein directly from the extract of gizzard myofibrils and then is suitable for the study of myosin light chain kinase in small muscles. The protein was judged to be greater than 95% pure by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The enzyme retains its activity since it catalyzes the calcium-calmodulin-dependent phosphorylation of the 20,000-Da myosin light chain. |
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