Sequence homologies between the tryptophanyl tRNA synthetases of Bacillus stearothermophilus and Escherichia coli. |
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Authors: | G P Winter B S Hartley A D McLachlan M Lee K H Muench |
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Affiliation: | Institut de Chimie des Substances Naturelles, CNRS, 91190 Gif sur Yvette, France;Laboratoire de Biologie et de Génétique Evolutives, CNRS, 91190 Gif sur Yvette France |
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Abstract: | Subcultures of ovaries and testis of the crab Carcinus maenas have been performed in the presence of L-[Me-14C]methionine. Introduction in the medium of a chromatographically-purified liposoluble fraction from the androgenic glands of the same animal inhibits the biological methylation of the tRNA of the ovaries by 62%. The inhibition of methylation of five individual bases varies from 45% to 84%. No inhibition of tRNA methylation is observed under the same conditions with testis subcultures. |
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