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Circadian rhythm of neurotensin levels in rat small intestine
Authors:C F Ferris  J K George  H E Albers
Affiliation:1. Servicio de Endocrinología y Nutrición, Complexo Hospitalario Universitario, Vigo, España;2. Servicio de Endocrinología y Nutrición, Complejo Asistencial de Zamora, Hospital Virgen de la Concha, Zamora, España;1. Department of Neurology, Kaohsiung Medical University Hospital, 100, Tzyou 1st Rd., Kaohsiung City, 80754, Taiwan;2. Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, No. 100, Shih-Chuan 1st Road, Kaohsiung City, 80708, Taiwan;3. Department of Neurology, College of Medicine, Kaohsiung Medical University, No. 100, Shih-Chuan 1st Road, Kaohsiung City, 80708, Taiwan;4. Department of Neurology, Health and Welfare Ministry Pingtung Hospital, No. 270, Ziyou Rd., Pingtung City, Pingtung County, 900, Taiwan;5. Department of Neurology, Kaohsiung Municipal Siaogang Hospital, No. 482, Shanming Rd., Siaogang Dist., Kaohsiung City, 812, Taiwan
Abstract:The present studies were undertaken to determine whether a 24 h rhythm occurs in neurotensin (NT) levels in the small intestine of the rat and if so, whether the rhythm depends upon the 24 h cycles of light or feeding. A total of 145 male rats were sacrificed at 4 h intervals and the levels of neurotensin-like immunoreactivity (NTLI) in the middle 30 cm of small intestine were determined by radioimmunoassay with region specific antisera. There was a significant (P less than 0.05) 24 h rhythm in the levels of NTLI in groups of rats maintained under constant illumination or a 12:12 light:dark cycle and fasted for either 24 h or provided food ad libitum. Levels of NTLI ranged from 50 to 140 pm/g and were highest during the early morning (0400-0800 h) and lowest during the afternoon (1200-1600 h). The NTLI from samples taken at 0400 and 1600 h was subjected to high-performance liquid chromatography. The levels of chromatographically and immunochemically characterized NT were consistent with the levels of NTLI, evidence that the 24 h variation in NTLI most likely reflects changes in the intestinal content of NT and not other substances with similar immunochemical properties.
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