| PTSD样情感行为异常大鼠杏仁核Caspase 9的改变 |
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| 引用本文： | 肖冰,韩芳,石玉秀. PTSD样情感行为异常大鼠杏仁核Caspase 9的改变[J]. 中国组织化学与细胞化学杂志, 2010, 19(1): 6-10 |
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| 作者姓名： | 肖冰 韩芳 石玉秀 |
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| 作者单位： | 中国医科大学基础医学院组织学与胚胎学教研室,沈阳,110001 |
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| 基金项目： | 国家自然科学基金资助项目(30600341) |
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| 摘 要： | 目的观察创伤后应激障碍(PTSD)样行为异常大鼠杏仁核神经元Caspase 9表达变化,有望揭示PTSD的部分发病机制。方法采用国际认定的SPS方法刺激建立大鼠PTSD模型,取成年健康雄性Wistar大鼠60只,随机分为SPS模型的1d、4d、7d组及正常对照组,采用免疫荧光法、免疫印迹和逆转录-聚合酶链式反应检测大鼠杏仁核Caspase 9的表达变化。结果SPS刺激后大鼠杏仁核神经元细胞内Caspase 9于1d开始逐渐升高,7d时表达最多;Caspase 9 mRNA的变化与之相一致。结论海马Caspase 9的表达变化,可能是PTSD大鼠情感行为异常的重要病理生理基础之一。
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| 关 键 词： | 创伤后应激障碍 杏仁核 Caspase 9 免疫印迹 逆转录-聚合酶链式反应 |
CHANGES OF CASPASE 9 IN AMYGDALA OF RATS WITH POSTTRAUMIC STRESS DISORDER-LIKE BEHAVIOR |
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| Xiao Bing,Han Fang,Shi Yuxiu. CHANGES OF CASPASE 9 IN AMYGDALA OF RATS WITH POSTTRAUMIC STRESS DISORDER-LIKE BEHAVIOR[J]. Chinese Journal of Histochemistry and Cytochemistry, 2010, 19(1): 6-10 |
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| Authors: | Xiao Bing Han Fang Shi Yuxiu |
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| Affiliation: | Department of Histology and Embryology;Basic Medical College;China Medical University;Shen yang 110001;China |
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| Abstract: | Objective To observe the changes of caspase 9 in the amygdala neurons of PTSD rats in order to reveal part of the pathogensis. Methods The SPS-method was used to set up the rat PTSD models. A total of 60 male Wistar rats were randomly divided into 1d,4d,7d groups of SPS and the normal control group. The expression of caspase 9 was detected by using immunofluorescence, western blot and RT-PCR. Results The expression of caspase 9 in the amygdala gradually increased at 1d after SPS, and reached the maximum at ... |
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| Keywords: | Posttraumatic stress disorder Amygdala Caspase 9 Western blot RT-PCR |
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