Temperature dependence of delayed chlorophyll fluorescence in intact leaves of higher plants. A rapid method for detecting the phase transition of thylakoid membrane lipids |
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Authors: | Michel Havaux Robert Lannoye |
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Institution: | (1) Laboratoire de Physiologie végétale, Université Libre de Bruxelles, 28 avenue Paul Héger, 1050 Bruxelles, Belgium |
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Abstract: | The temperature dependence of the yield of in vivo prompt and delayed chlorophyll fluorescence was investigated in maize and barley leaves. In the chilling-sensitive maize, delayed fluorescence at steady-state level showed a maximum near the temperature at which thylakoid membrane lipids undergo a phase transition as revealed by differential scanning calorimetry measurements. In the chilling-resistant barley, no phase transition was detected above 0°C and the delayed light emission varied in a monotonic fashion. It was shown that measurements of delayed luminescence intensity in vivo can provide a rapid and sensitive method for detecting the phase change of membrane lipids in intact leaves of chilling-sensitive plant species such as tomato, cotton, cucumber, castor bean or avocado. In contrast, the use of steady-state prompt chlorophyll fluorescence as an indicator of membrane fluidity change was not successful. |
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Keywords: | chilling sensitivity delayed light emission prompt chlorophyll fluorescence differential scanning calorimetry |
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