Cytochrome b of the Dictyostelium discoideum plasma membrane |
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Authors: | Ingrid Glomp and Benno Hess |
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Affiliation: | Max-Planck-Institut für Ernährungsphysiologie, Rheinlanddamm 201, 4600, Dortmund 1, F.R.G. |
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Abstract: | Cytochrome b of the plasma membrane of Dictyostelium discoideum was investigated in purified plasma membranes and in solubilized form. The membrane-bound cytochrome b can be reduced by NADH. This reduction is inhibited by p-hydroxymercuribenzoate. The reduced cytochrome b does not react with carbon monoxide. Its apparent molecular weight lies between 13000 and 16000. Tryptic digestion yields a large, heme-containing peptide with an apparent molecular weight between 12000 and 15000. After solubilization with cholate, cytochrome b can be enriched by reversed-phase HPLC, indicating that it contains also a hydrophobic component. With these properties, cytochrome b of the D. discoideum plasma membrane resembles microsomal cytochrome b5. |
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Keywords: | Cytochrome b Plasma membrane Electron transport (D. discoideum) |
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