Structure and analysis of phospholipase D gene from Ricinus communis L |
| |
Authors: | Liwen Xu Ling Zheng Sean J Coughlan Xuemin Wang |
| |
Institution: | (1) Department of Biochemistry, Kansas State University, 66506 Manhattan, KS, USA;(2) Pioneer Hi-Bred International Inc., 50131 Johnston, IA, USA;(3) Present address: Department of Diagnostic Medicine/Pathology, Kansas State University, 66506 Manhattan, KS, USA |
| |
Abstract: | Phospholipase D (PLD; EC 3.1.4.4) has been proposed to play a pivotal role in various cellular processes, but molecular understanding of this enzyme is rather limited. This report describes the nucleotide sequence, structure, and genomic organization of a PLD gene from castor bean (Ricinus communis L. cv. Hale). The PLD gene was isolated from a castor bean genomic library using the PLD cDNA as a hybridization probe. Sequence comparison with the PLD cDNA revealed that the PLD gene consisted of four exons and three introns, one of which interrupts the 5-untranslated region. Southern blot analysis indicated that the cloned PLD gene was present as a single-copy gene, and yet there were other PLD or PLD-related sequences in the castor bean genome. |
| |
Keywords: | castor bean gene cloning phospholipid hydrolysis |
本文献已被 SpringerLink 等数据库收录! |
|