| 固定化金属螯合亲和膜纯化重组抗菌肽研究 |
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| 引用本文： | 魏琪,姚汝华,鲍时翔.固定化金属螯合亲和膜纯化重组抗菌肽研究[J].生物化学与生物物理进展,2000,27(4):401-403. |
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| 作者姓名： | 魏琪 姚汝华 鲍时翔 |
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| 作者单位： | 1. 华南理工大学生物工程系,广州,510641
2. 中国热带农业科学院生物技术国家重点实验室,海口,571101 |
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| 基金项目： | 国家自然科学基金资助项目(29606004). |
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| 摘 要： | 用自行制备的固定化金属螯合亲和膜对N端含六个组氨酸标记的重组抗菌肽进行了分离纯化，并较好地解决了金属离子泄露问题.实验表明，固定化金属螯合亲和膜性能优于传统琼脂糖凝胶介质，完全适用于分离纯化含有多组氨酸标记的重组蛋白质.
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| 关 键 词： | 亲和层析，亲和膜，抗菌肽 |
| 收稿时间： | 9/7/1999 12:00:00 AM |
| 修稿时间： | : |
Separate Recombinant Antibacterial Peptide With Immobilized Metal-chelated Affinity Chromatography Membranes |
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| WEI Qi,YAO Ru-Hua and BAO Shi-Xiang.Separate Recombinant Antibacterial Peptide With Immobilized Metal-chelated Affinity Chromatography Membranes[J].Progress In Biochemistry and Biophysics,2000,27(4):401-403. |
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| Authors: | WEI Qi YAO Ru-Hua and BAO Shi-Xiang |
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| Abstract: | Immobilized metal-chelated affinity chromatography(IMAC) membranes were prepared for separating a recombinant fusion antibacterial peptide,which carried a polyhistidine sequence (HIS6-tag) at the N-terminus. Low bleeding of metal ion was achieved. It was proved that the properties of IMAC membranes were better than conventional chelating sepharose fast flow column. The fractionation of recombinant proteins that carry a polyhistidine tag is currently perhaps the most promising application of IMAC. |
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| Keywords: | affinity chromatography affinity membrane antibacterial peptide |
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