Determination of gamma-aminobutyric and glutamic acids in rat brain by liquid chromatography with electrochemical detection |
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| Authors: | S M Lasley R D Greenland I A Michaelson |
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| Institution: | 1. Department of Pharmacology, Texas College of Osteopathic Medicine, Fort Worth, TX 76107, USA;2. Department of Environmental Health, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA |
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| Abstract: | The determination of glutamic and gamma-aminobutyric acids in rat brain regions by derivatization with o-phthaldialdehyde-thiol, isocratic separation by liquid chromatography, and quantification by electrochemical detection provides a simple and precise method for assessing changes in glutamatergic and GABAergic neuronal systems. Gamma-aminobutyric acid was eluted in 30-35 minutes followed by a washout step with 90% methanol to remove all amino acid derivatives with longer retention times. Homoserine was used as an internal standard. Significant increases in gamma-aminobutyric acid content in nucleus accumbens and substantia nigra could be detected 20 minutes after injection of 400 mg/kg valproic acid. |
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| Keywords: | Send reprint requests to: Dr Stephen M Lasley Department of Pharmacology Texas College of Osteopathic Medicine Camp Bowie at Montgomery Fort Worth TX 76107 U S A |
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