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Characteristics of androgen binding in rat adrenal tissue using [3H]methyltrienolone (R1881) as tracer |
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| Authors: | J Asselin R Melancon |
| Institution: | Laboratory of Molecular Endocrinology, Le Centre Hospitalier de l''Université Laval, Quebec G1V 4G2, Canada |
| Abstract: | A high level of binding of 3H]methyltrienolone (R1881 = 17beta-hydroxy-17alpha-methyl-estra-4, 9, 11-trien-3-one) was found in cytosol prepared from adrenals of castrated male rats. Binding of 3H]R1881 was of high affinity (DK = 6.2 nM) and highly specific for androgens. The 3H]R1881 complex migrates at 7-9S on sucrose gradients in low ionic strength buffer and at 4-5S in buffer containing 0.4M KC1. All binding studies have been performed in parallel with rat ventral prostate and adrenal cytosol. The present data suggest the presence of an androgen binding component in rat adrenal tissue. |
| Keywords: | R1881 methyltrienolone 17β-hydroxy-17α-methy1-estra-4 9 11-trien-3-one DHT androstanolone 5α-dihydrotestosterone 17β-hydroxy-5α-androstan-3-one R2956 2α 2β 17α-trimethyl-17β hydroxy-4 9 11-estratriene-3-one R5020 17 21-dimethyl-19-nor-4 9-pregnadiene-3 20-dione dexamethasone 11β 17 21-trihydroxy-9-fluoro-16αmethyl-1 4-pregnadiene-3 20-dione cyproterone acetate 1 2α-methy1ene-6-chloro-17α-acetoxy-4 6-pregnadiene-3 20-dione Flutamide 4-nitro-3'-trif1uoromethylisobutyran 12i1ide triamcinolone acetonide 9-fluoro-11β 21-di hydroxy-16α 17-isopro-pylidenedioxy-1 4-pregnadiene-3 20-dione D-Norgestrel 13-ethyl-17-ethinyl-17β-hydroxy-4-gonen-3-one Provera 17α-acetoxy-6α-methylprogesterone epitestosterone 17α-hydroxy-4-androsten-3-one |
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