The behaviour of tomato golden mosaic virus DNA in cultured cells isolated from systemically infected tobacco leaves |
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| Authors: | M. J. Slomka K. W. Buck R. H. A. Coutts |
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| Affiliation: | (1) Department of Pure and Applied Biology, Imperial College of Science and Technology, Prince Consort Road, SW7 2BB London, UK;(2) Present address: Department of Medical Microbiology, London School of Hygiene and Tropical Medicine, Keppel Street, WC1E 7HT London, UK |
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| Abstract: | When callus tissue was cultured from leaf pieces taken from a Nicotiana tabacum cv. Xanthi nc. plant systemically infected with tomato golden mosaic virus (TGMV), TGMV-specific DNA persisted for up to 6 months in culture. Analysis of TGMV-specific intracellular DNA forms indicated a decrease in double-stranded relative to single-stranded forms and an increase in sub-genomic relative to genomic single-stranded DNA species in the callus tissue compared to those in the original leaf explant. The implications of the results with regard to TGMV replication are discussed. |
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