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Elevated F2-isoprostanes in thalassemic patients |
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| Authors: | Matayatsuk Chutima Lee Chung-Yung J Kalpravidh Ruchaneekorn W Sirankapracha Pornpan Wilairat Prapin Fucharoen Suthat Halliwell Barry |
| Affiliation: | aFlow Innovation-Research for Science and Technology Laboratories (First Labs), Mahidol University, Rama 6 Rd, Bangkok 10400, Thailand bDepartment of Chemistry, Faculty of Science, Mahidol University, Rama 6 Rd, Bangkok 10400, Thailand cNational University of Singapore, Yong Loo Lin School of Medicine, Department of Biochemistry, 8 Medical Drive, Singapore 117597, Singapore dDepartment of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Prannok Rd., Bangkoknoi, Bangkok, 10700, Thailand eThalassemia Research Center, Institute of Science and Technology for Research and Development, Mahidol University (Salaya Campus), Nakronpathom, Thailand |
| Abstract: | This study was aimed at investigating oxidative stress in thalassemic patients by measurement of the oxidative damage biomarker, F2-isoprostanes (F2-IsoPs), using gas chromatography-mass spectrometry. The results showed that the mean value of urinary F2-IsoPs, normalized with creatinine, in the thalassemic group was significantly higher than that from healthy subjects (3.38 ± 2.15 ng/mg creatinine vs 0.86 ± 0.55 ng/mg creatinine, respectively), and the mean value of plasma total F2-IsoPs in the thalassemic group was also significantly higher than that from healthy subjects (0.39 ± 0.15 ng/ml vs 0.18 ± 0.03 ng/ml, respectively). Serum ferritin, erythrocyte superoxide dismutase (SOD), glutathione peroxidase, glutathione, and TBARS levels after treatment of erythrocytes with H2O2 were also investigated, and serum ferritin and erythrocyte SOD levels were significantly higher in thalassemic patients. Our findings are consistent with oxidative stress in thalassemia patients. |
| Keywords: | F2-isoprostanes Oxidative stress Thalassemia |
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