Nucleosome cores containing H2B,H3, H4 and HMG2 are reconstituted |
| |
Authors: | Kazuei Mita Mitsuo Zama Sachiko Ichimura Koei Hamana |
| |
Institution: | 1. Division of Chemistry, National Institute of Radiological Sciences, Chiba, Chiba 260, Japan;2. College of Medical Care and Technology, Gunma University Maebashi, Gunma 371, Japan |
| |
Abstract: | Nucleosome cores mixed with the high mobility group proteins, HMG1 and HMG2, in 2 M NaCl, 5 M urea, 0.2 mM EDTA and 10 mM Tris pH 7.0, have been reconstituted by salt gradient dialysis. The reconstituted material, in 10 mM Tris pH 7.0, had a sedimentation peak at the same position as that of control nucleosome cores in sucrose density gradient ultracentrifugation. The SDS polyacrylamide gel electrophoresis of the reconstituted nucleosome cores demonstrated that they contain H2B, H3, H4 and HMG2 and are selectively deficient in H2A. The circular dichroism of DNA of the reconstituted cores was indistinguishable from that of control nucleosome cores. The results suggest that HMG2 replaces H2A as a component of the nucleosome histone core during reconstitution. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|