Proline synthesis and redox regulation: Differential functions of pyrroline-5-carboxylate reductase in human lymphoblastoid cell lines |
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Authors: | Georges Lorans James M. Phang |
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Affiliation: | 1. Endocrine Section, Metabolism Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20205 USA |
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Abstract: | Pyrroline-5-carboxylate (P5C) reductase not only catalyzes the final step in proline biosynthesis but also mediates redox regulation. We examined these dual functions in human lymphoblastoid cell lines (REH, LHN13) one of which (REH) is deficient in proline synthesis. In spite of the deficiency in proline synthesis in REH, the PC-mediated redox effect was relatively intact. The dissociation of these functions was due to qualitative as well as quantitative differences in the REH enzyme relative to its utilization of NADPH and NADH. |
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Keywords: | To whom reprint requests should be sent. |
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