Ribonucleic acid-protein crosslinking in Escherichia coli ribosomal 30S subunits by the use of two new heterobifunctional reagents: 4-azido-2,3,5,6-tetrafluoropyridine and 4-azido-3,5-dichloro-2,6-difluoropyridine |
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| Authors: | Régine Millon Jean-Pierre Ebel François Le Goffic Bernard Ehresmann |
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| Affiliation: | 1. Laboratoire de Biochimie, Institut de Biologie Moléculaire et Cellulaire du C.N.R.S., 15, rue René Descartes, 67000-Strasbourg, France.;1. Groupe des Laboratoires du C.N.R.S., Centre d''Etude et de Recherche de Chimie Organique Appliquée, 2–8, rue Henri Dunant, 94300-Thiais, France. |
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| Abstract: | Two new heterobifunctional reagents (4-azido-2,3,5,6-tetrafluoropyridine and 4-azido-3,5-dichloro-2,6-difluoropyridinel were synthesized and used to crosslink RNA to protein in Escherichia coli ribosomal 30S subunits. The maximal yield of crosslinking of the protein moiety was evaluated as 3.5%. Only proteins S4, S7 and S9 were found to be crosslinked to the 16S RNA within the 30S subunits. |
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| Keywords: | To whom correspondence should be addressed. |
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