Monoclonal antibodies against ovalbumin

Fusion of cells of the NS-1 mouse myeloma line with spleen cells from $\text{BALB}\text{c}$ mice immunized against ovalbumin produced hybrid cells which continuously secrete antibodies specific for ovalbumin. One of these cells was used to establish a cloned line. Studies of its antibody obtained either from ascites fluid or from medium from hybridoma cultures showed high titer and specificity against ovalbumin using the double antibody technique with rabbit anti-mouse immunobeads; the antibody proved to be of the IgG₁ (kappa) subclass and type.