Synthesis of D-cysteine from 3-chloro-D-alanine and hydrogen sulfide by 3-chloro-D-alanine hydrogen chloride-lyase (deaminating) of <span class="math"><math><mtext>Pseudomonas</mtext><mtext>putida</mtext></math></span>期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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Synthesis of D-cysteine from 3-chloro-D-alanine and hydrogen sulfide by 3-chloro-D-alanine hydrogen chloride-lyase (deaminating) of $Pseudomonas putida$

Authors:	Hideaki Yamada Toru Nagasawa Haruyuki Ohkishi Bunsei Kawakami Yoshiki Tani

Institution:	1. Department of Agricultural Chemistry, Kyoto University, Kyoto 606, Japan.;1. Central Research Laboratory, Mitsubishi Chemical Industries, Yokohama 227, Japan.

Abstract:	Synthesis of D-cysteine from 3-chloro-D-alanine and hydrogen sulfide is catalyzed by highly purified 3-chloro-D-alanine hydrogen chloride-lyase from $Pseudomonas putida$ . The synthetic reaction proceeds optimally at pH 8.5, as a function of enzyme concentration and incubation time. The enzymatically synthesized D-cysteine was isolated from the large scale reaction mixture and identified by physicochemical means.

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