Cell-free synthesis of angler fish preproinsulin: Complete amino acid sequence of the signal peptide |
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Authors: | Dennis Shields |
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Institution: | Department of Anatomy Albert Einstein College of Medicine 1300 Morris Park Avenue Bronx, N.Y. 10461 USA |
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Abstract: | Messenger ribonucleic acid isolated from angler fish () islets of Langerhans was translated in the wheat germ cell-free protein synthesizing system containing different combinations of radioactive amino acids. Preproinsulin (~ 11,000 daltons) was identified amongst the translation products, by sodium dodecyl sulfate gel electrophoresis, and subjected to microsequencing techniques. The fish preproinsulin was found to possess an NH2-terminal signal peptide of 24 amino acids, with regions of homology to human, rat and chicken preproinsulin signal sequences. |
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Keywords: | SDS sodium dodecyl sulfate E R endoplasmic reticulum |
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