The study of two alkaliphilic thermophile bacteria of the <Emphasis Type="Italic">Anoxybacillus</Emphasis> genus as producers of extracellular proteinase |
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Authors: | E V Lavrenteva A P Shagzhina O B Babasanova Y E Dunaevsky Z B Namsaraev D D Barkhutova |
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Institution: | 1.Institute of General and Experimental Biology, Siberian Branch,Russian Academy of Sciences,Ulan-Ude, Buryat Republic,Russia;2.Belozersky Institute of Physicochemical Biology,Moscow State University,Moscow,Russia;3.Winogradsky Institute of Microbiology,Russian Academy of Sciences,Moscow,Russia |
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Abstract: | Two strains of alkaliphilic thermophile bacteria of the genus Anoxybacillus from hydrothermal vents of Lake Baikal were detected and characterized. It was demonstrated that proteinases secreted by
these bacteria had wide substrate specificity, hydrolyzed proteins and n-nitroanilide substrates, and showed maximal activity at pyroglutamyl-alanine-alanine-leucine n-nitroanilide hydrolysis. We determined maximal activity of the proteinases at alkaline pH values (10.0–10.5), the enzymes
were thermostable and were characterized by a wide thermal optimum (55–70°C). The results of inhibitor analysis and substrate
specifity examination of extracellular enzymes demonstrated their belonging to the subtilisin-like serine proteinases. |
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