Aptameric enzyme subunit for homogeneous DNA sensing |
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Authors: | Kazunori Ikebukuro Wataru Yoshida Koji Sode |
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Institution: | (1) Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan |
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Abstract: | We have developed an aptameric enzyme subunit (AES) which can detect the DNA in a homogeneous solution. The AES is an artificial
enzyme subunit composed of an enzyme-inhibiting aptamer bearing a target-molecule binding site. We connected a probe DNA to
a thrombin-inhibiting aptamer at its 5′ or 3′ end. The inhibitory activity of the thrombin-inhibiting aptamer bearing the
probe DNA decreased compared to that of the original aptamer; however, it recovered upon hybridization with the target DNA.
Using this AES, we were able to detect target DNAs by measuring the thrombin activity in a homogeneous solution.
K. Ikebukuro and W. Yoshida have contributed equally to this work. |
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Keywords: | Aptameric enzyme subunit Enzyme-inhibiting aptamer Homogeneous DNA sensing |
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