Tissue culture response of Beta germplasm: callus induction and plant regeneration |
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| Authors: | J Abe H Nakashima K Mitsui T Mikami Y Shimamoto |
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| Institution: | (1) Laboratory of Industrial Crops, Faculty of Agriculture, Hokkaido University, 060 Sapporo;(2) Experiment Farms, Faculty of Agriculture, Hokkaido University, 060 Sapporo, Japan;(3) Present address: Advanced Technology Research Laboratories, Sumitomo Metal Industries, Ltd, 289-06 Ibaraki, Japan |
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| Abstract: | Callus cultures of 18 sugarbeet (Beta vulgaris) lines, two accessions of B. maritima and a B. macrocarpa accession were initiated from aseptically germinated seeds. Plant regeneration through organogenesis was obtained either on MS or B5 medium containing various concentrations and combinations of naphthaleneacetic acid (NAA), 6-benzylaminopurine (BAP), 2,3,5-triiodobenzoic acid (TIBA) and abscisic acid (ABA). Genotypes differed in their abilities of callus formation and regeneration: seven out of 18 sugarbeet lines, and an accession of B. maritima were capable of regenerating plantlets. Our data also indicated that 2  M TIBA promoted morphogenesis from callus culture in the presence of 5 M BAP. |
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| Keywords: | Beta macrocarpa Beta maritima Beta vulgaris genotype morphogenic response |
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