Characterizing HMW-GS alleles of decaploid <Emphasis Type="Italic">Agropyron elongatum</Emphasis> in relation to evolution and wheat breeding |
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Authors: | Shuwei Liu Xin Gao Guangmin Xia |
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Institution: | (1) School of Life Sciences, Shandong University, 250100 Jinan, People’s Republic of China |
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Abstract: | Bread wheat quality is mainly correlated with high molecular weight glutenin subunits (HMW-GS) of endosperm. The number of
HMW-GS alleles with good processing quality is limited in bread wheat cultivars, while there are plenty of HMW-GS alleles
in wheat-related grasses to exploit. We report here on the cloning and characterization of HMW-GS alleles from the decaploid
Agropyron elongatum. Eleven novel HMW-GS alleles were cloned from the grass. Of them, five are x-type and six y-type glutenin subunit genes.
Three alleles Aex4, Aey7, and Aey9 showed high similarity with another three alleles from the diploid Lophopyrum elongatum, which provided direct evidence for the Ee genome origination of A. elongatum. It was noted that C-terminal regions of three alleles of the y-type genes Aey8, Aey9, and Aey10 showed more similarity with x-type genes than with other y-type genes. This demonstrates that there is a kind of intermediate
state that appeared in the divergence between x- and y-type genes in the HMW-GS evolution. One x-type subunit, Aex4, with an additional cysteine residue, was speculated to be correlated with the good processing quality of wheat introgression
lines. Aey4 was deduced to be a chimeric gene from the recombination between another two genes. How the HMW-GS genes of A. elongatum may contribute to the improvement of wheat processing quality are discussed. |
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