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Endo-PG诱导培养小麦细胞PGIP产生的研究
引用本文:周立,李建吾,郑远旗,余露.Endo-PG诱导培养小麦细胞PGIP产生的研究[J].中国生物化学与分子生物学报,1997,13(3):365-367.
作者姓名:周立  李建吾  郑远旗  余露
作者单位:四川大学生物系!成都,610064,四川大学生物系!成都,610064,四川大学生物系!成都,610064,中国科学院成都生物所!成都,610041
摘    要:内切多聚半乳糖醛酸酶(endo-polygalacturonase,endo-PG)是待异水解细胞壁成分多聚半乳糖醛酸的酶,水解产生的10~13个糖基的寡聚半乳糖醛酸片段是活性诱导因子,激活植物自身防御系统.我们已研究发现单子叶植物小麦中存在多聚半乳糖醛酸酶抑制蛋白(polygalacturonaseinhibitingprotein,PGIP),并已将其分离纯化,对其性质作了初步研究1,2]文献报导3]PGIP是在未分化的细胞中合成的.本文报导在悬浮培养的小麦细胞中加入Endo-PG观察其PGIP的生成,比较赤霉病的高抗品种与低抗品种中PGIP的合成情况,探讨PGIP与植物防御作…

收稿时间:1997-06-20

Polygalacturonase Inhibiting Protein Production in Cultured Wheat Cells Induced by Endo-polygalacturonase
Zhou Li,Li Jian-Wu,Zheng Yuan-Qi.Polygalacturonase Inhibiting Protein Production in Cultured Wheat Cells Induced by Endo-polygalacturonase[J].Chinese Journal of Biochemistry and Molecular Biology,1997,13(3):365-367.
Authors:Zhou Li  Li Jian-Wu  Zheng Yuan-Qi
Institution:(Department of Biology, Sichuna University, Chengdu 610064)Yu Lu (Chengdu Institute of Biology, Academia Sinica,Chengdu 610041
Abstract:The activity of polygalacturonase inhibiting protein (PGIP)was assayed in four kind of cultures induced endo-polygalacturonase (endo-PG). It was discovered that endo-PG stimulated obviously the accumulation of PGIP. The PGIP contents of SuMai No. 3 and SA-Ⅱ cells,which are fungal disease-resistant,are higher than the contents of Mian Yang No. 11 and JingHua No.1. It is indicated in cell level that PGIP has relation to resistance of plant.
Keywords:Polygalacturonase inhibiting protein  Cell culture  undo-polygalacturonase
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