Isolation and characterization of culturable halophilic microorganisms of salt ponds in Lianyungang,China |
| |
Authors: | Hongyu Wen Liu Yang Lulu Shen Bin Hu Zongyun Li Qijiang Jin |
| |
Institution: | (1) School of Life Science, Xuzhou Normal University, 221116 Xuzhou, China |
| |
Abstract: | The Lianyungang salt ponds are an extreme saline environment, and their microbial communities have not been characterized.
A typical extreme halophilic archaeon strain designated as HBCC-2 (GenBank accession number: EF687739) was isolated from the
salt ponds of Lianyungang in Jiangsu Province, P. R. China, using conventional microbial culture methods. The other halotolerant
bacterial strain designated as HBCC-3 (GenBank accession number: EU377478) was isolated from the same sampling sites. The
morphological and physiological characteristics of HBCC-2 and HBCC-3 were observed and examined. G+C content of HBCC-2 and
HBCC-3 were determined using high-performance liquid chromatography. The cellular phospholipid fatty acids were analyzed using
gas chromatography-mass spectrometry. The 16S rRNA gene sequences of the strains HBCC-2 and HBCC-3 were amplified by PCR using
archaeal primers and bacterial primers, respectively. Homology of 16S rRNA gene sequences of the strains HBCC-2 and HBCC-3
were compared with the other similar sequences obtained from GenBank using the BLAST program. Phylogenetic analysis was performed
using the software MEGA 4.0 after multiple alignments of sequence data using software CLUSTALW 1.8. The evolutional distances
(by Kimura’s model) were calculated and the clusters were performed with the neighbor-joining method. The results showed that
the 16S rRNA gene sequences of the strains HBCC-2 and HBCC-3 are related to the genera Halorubrum and Alkalibacillus, respectively. Two phylogenetic trees were constructed by phylogenetic analysis based on the 16S rRNA gene sequence. Based
on the above results, the strains HBCC-2 and HBCC-3 were finally identified. The discovery of the two species provides an
opportunity to further study these halophilic microorganisms in the Lianyungang salt ponds. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|