Monoclonal antibody analysis of skin in chronic murine graft vs host disease produced across minor histocompatibility barriers |
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| Authors: | R Giorno K L Choi H R Katz H N Claman |
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| Institution: | 1. Division of Allergy and Clinical Immunology, Department of Medicine, University of Colorado Health Sciences Center, Container B-164, Denver, Colorado 80262, USA;1. Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115 USA;2. The Department of Rheumatology and Immunology, Brigham and Womens Hospital, Boston, Massachusetts 02115 USA;1. Food Science Program, University of Missouri, Columbia, MO 65211, USA;2. Department of Biomedical Engineering, University of Connecticut, Storrs, CT 06269, USA;1. Laboratory for Stem Cell Therapy, Faculty of Medicine, Tsukuba University, Tsukuba 305-8577, Japan;2. Division of Stem Cell Biology, Center for Stem Cell Therapy, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan;3. MRC Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford, Oxford OX3 9DS, UK;4. Division of Malaria Immunology and International Vaccine Design Center, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan;5. Department of Pathology, Research Hospital, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan;6. International Research Center for Medical Sciences, Kumamoto University, Kumamoto City 860-0811, Japan;7. Pharma Solutions, Nutrition & Health, BASF SE, Carl-Bosch-Strasse 38, 67056 Ludwigshafen am Rhein, Germany;8. Pharma Solutions, Nutrition & Health, BASF Japan Ltd, Tokyo 103-0022, Japan;9. Tokyo Medical and Dental University, Tokyo 113-8510, Japan;10. York Biomedical Research Institute, Department of Biology, University of York, Wentworth Way, York YO10 5DD, UK |
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| Abstract: | Chronic graft vs host disease (GVHD) across minor histocompatibility barriers was produced in BALB/c mice by the injection of spleen cells from B10.D2 mice. Changes in the skin were analyzed in frozen sections using a panel of monoclonal antibodies detected by immunoperoxidase methods. Compared to control animals, a number of changes occurred in the skin of animals with chronic GVHD. In the epidermis, there were increased numbers of Thy-1-positive dendritic cells; keratinocytes expressed Thy-1 and Ia antigens. T lymphocytes appeared in both dermis and epidermis. In the early stages, cells with "helper" and "suppressor" phenotypes were present, while at later times "helper" cells remained in the epidermis and "suppressor" cells remained in the dermis. Cells bearing markers of macrophages were prominent in both dermis and epidermis after the second week. Of great interest was the appearance of spindle-shaped cells in the dermis which expressed Thy-1 and Ia. These cells resembled fibroblasts which may be activated to produce the excess collagen seen in the skin of chronic GVHD. |
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