Hexose and Glycerol Absorption by Some Trypanosomatidae

SYNOPSIS. Culture forms of 9 species of Trypanosomatidae were studied. Of these, Crithidia fasciculata, Leishmania brasiliensis, L. donovani, L. tropica, Trypanosoma conorhini, T. cruzi and T. rangeli consumed significantly less glycerol than glucose, both when the 2 substrates were offered singly or simultaneously. On the other hand, T. gambiense and T. rhodesiense consumed as much glycerol as glucose when the 2 substrates were given separately. When both substrates were offered simultaneously, more glycerol than glucose was consumed, but the sum of glucose + glycerol carbon taken up approximated closely that taken from glucose alone. This held for species consuming only little glycerol, indicating that in all cases some mutual inhibition took place. Atttempts to adapt T. cruzi to glycerol consumption were unsuccessful. When T. gambiense and T. rhosiense were cultivated in the presence of glycerol, their normrapid glycerol consumption remained also unchanged but, curiothey consumed more glucose than before "adaptation." Iodoacetami and NaF strongly inhibited glucose and glycerol absorption of 7 gambiense ; phloridzin, deoxygalactose and deoxyglucose gave nonificant inhibition. KCN slightly stimulated glucose absorption, weakly inhibited glycerol absorption. Omission of sodium from medium was without effect, while lack of CO₂ markedly inhiglucose and glycerol uptake. Galactose was not a satisfactory sub strate for T. gambiense and T. rhodesiense , nor were deoxygalactos and deoxyglucose. These 3 hexoses were consumed in small amonly and did not allow maintenance of motility.