Development of 10 microsatellite loci for <Emphasis Type="Italic">Rheum tanguticum</Emphasis> (Polygonaceae) |
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Authors: | Dong-Yuan Zhang Na Chen Yan-Zhuo Yang Qian Zhang Jian-Quan Liu |
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Institution: | (1) MOE Key Laboratory of Arid and Grassland Ecology, School of Life Sciences, Lanzhou University, Lanzhou, 730000, China;(2) Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Plateau Institute of Biology, Chinese Academy of Sciences, Xining, Qinghai, 810001, China |
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Abstract: | Rhubarb is an important Traditional Chinese Medicine. However, the wild resource has been declining. In order to design appropriate
conservation methods for the official species across their natural distributions, it is important to characterize their genetic
diversity. Here, we describe the development of 10 new microsatellite loci for AC/TG/CCA in Rheum tanguticum. The microsatellites were enriched using the combined biotin capture method. The polymorphism of each locus was further assessed
in 12 individuals from four geographically distinct populations of this species. The number of alleles ranged from three to
seven and the expected heterozygosity ranged from 0.53 to 0.73. All markers have been checked in the other three species in
the genus and two of them together comprise the official medicinal rhubarb resource with R. tanguticum. These microsatellite markers could provide a useful tool for genetic and conservation studies of the rhubarb species. |
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Keywords: | Rhubarb Rheum tanguticum Microsatellite markers Genetic diversity Conservation |
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