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Robusta coffee beans post-harvest microflora: Lactobacillus plantarum sp. as potential antagonist of Aspergillus carbonarius |
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| Authors: | Djossou Olga Perraud-Gaime Isabelle Mirleau Fatma Lakhal Rodriguez-Serrano Gabriela Karou Germain Niamke Sebastien Ouzari Imene Boudabous Abdellatif Roussos Sevastianos |
| Affiliation: | aInstitut Méditerranéen d’Écologie et de Paléoécologie (IMEP, UMR CNRS/IRD 6116), Laboratoire d’Écologie Microbienne et Biotechnologies, FST St Jérôme, Université Paul Cézanne, 13397 Marseille Cedex 20, France;bUniversidad Autónoma Metropolitana Unidad Iztapalapa (UAM-I), Departamento de Biotecnología, Laboratorio de Fermentación en Medio Sólido, Avenida San Rafael Atlixco 186, Col. Vicentina, C.P. 09340 D.F. México, Mexico;cUniversité de Cocody, UFR Biosciences, 22 B.P. 582, Abidjan 22, Cote d’Ivoire;dLaboratoire de Microbiologie et Biomolécules actives, Université de Tunis El Manar, Faculté des sciences de Tunis, Tunisia |
| Abstract: | Coffee contamination by ochratoxigenic fungi affects both coffee quality as well as coffee price with harmful consequences on the economy of the coffee exporting countries for whom which is their main source of income. Fungal strains were isolated from coffee beans and identified as black Aspergilli. Ochratoxigenic moulds like Aspergillus carbonarius were screened and selected for detailed studies. Also lactic acid bacteria (LAB) were isolated from silage coffee pulp and their antifungal activity was tested on dual-culture agar plate. Ten of the isolated LAB demonstrated antifungal effect against A. carbonarius. API 50 CH and APIZYM were used to perform phenotypic identification. 16S rDNA sequencing was made to confirm the results. |
| Keywords: | Coffee robusta Aspergillus carbonarius Lactic acid bacteria Antagonism |
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