In Vivo Studies of the Biosynthesis of [alpha]-Eleostearic Acid in the Seed of Momordica charantia L |
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Authors: | Liu L Hammond E G Nikolau B J |
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Institution: | Department of Food Science and Human Nutrition (L.L.,E.G.H., B.J.N.) and Department of Biochemistry and Biophysics (E.G.H., B.J.N.), Iowa State University, Ames, Iowa 50011. |
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Abstract: | In vivo radiotracer experiments using 14C-labeled acetate, oleate, linoleate, and linolenate were conducted to investigate the biosynthesis of alpha]-eleostearic acid in the seeds of Momordica charantia. With the exception of 14C]linolenate, all of these precursors radioactively labeled alpha]-eleostearate. Kinetics of the time course of metabolism of the radioactive precursors indicate that linoleate is the acyl precursor of alpha]-eleostearate and that its conversion to alpha]-eleostearate occurs while the acyl moiety is esterified to PC. Pulse-chase experiments with 14C-labeled acetate or linoleate provided additional corroborative evidence that linoleoyl PC is the precursor of alpha]-eleostearoyl PC. |
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