Increase of the antimicrobial susceptibility ofYersinia enterocolitica associated with the expression of the virulence plasmid |
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Authors: | Dr. Maria Jimenez-Valera Alfonso Ruiz-Bravo Sergio M. Román Alberto Ramos-Cormenzana |
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Affiliation: | (1) Department of Microbiology, Faculty of Pharmacy, University of Granada, 18001 Granada, Spain |
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Abstract: | Virulent strains ofYersinia enterocolitica incubated in RPMI 1640 medium with 25 mM HEPES at 37°C were more susceptible to several antibiotics than their plasmid-free isogenic derivatives. The enumeration of viable bacteria in RPMI 1640 agar at 37°C to discriminate between plasmid-bearing and spontaneously derived, plasmid-free bacteria made it possible to show that the plasmid presence was associated with a fourfold decrease of minimal inhibitory concentration of ampicillin, streptomycin, gentamicin, chloramphenicol, and oxytetracycline. SinceY. enterocolitica is an intracellular pathogen and RPMI 1640 medium mimics the intracellular milieu, the plasmid-associated increase of susceptibility to antibiotics that are concentrated by animal cells may be of clinical relevance. |
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