Isolation and characterization of new bacteriophages for Myxococcus xanthus |
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| Authors: | F K Rodrigues Virpi Virrankoski-Castrodeza J H Parish K Grimm |
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| Institution: | (1) Department of Biochemistry, University of Leeds, 9 Hyde Terrace, LS2 9LS Leeds, UK;(2) Botanisches Institut I der Universität Karlsruhe, D-7500 Karlsruhe, Germany |
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| Abstract: | Bacteriophages for Myxococcus xanthus of similar morphology to phage Mx4 were isolated from cultures of a variety of myxobacterial species. Phages similar to Mx1 and Mx8 were obtained by infecting M. xanthus with one of the phages of the Mx4 group that had been treated with either UV light or a chemical mutagen.The DNA molecules from the phages were characterized by electron microscopy. One phage, Mx113, contains an unusual type of terminal redundancy revealed by examination of denatured and re-annealed DNA.Several of the phages of the Mx4 group and the other two new phages, Mx113 and Mx811, were found capable of transducing genetic markers in M. xanthus.One phage, Mx416, was characterized in more detail. It establishes true lysogens in M. xanthus; the phage plaques on both a non-motile mutant and also on a wild-type host although it is restricted in the latter.We dedicate this paper to Professor Dr. Hans Kühlwein in the year of his retirement and in recognition of his many contributions to the study of Myxobacteria |
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| Keywords: | Bacteriophage Myxococcus xanthus Transduction Lysogeny Restriction |
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