Gene Expression Profile of Hemocytes of Kuruma Shrimp, Marsupenaeus japonicus Following Peptidoglycan Stimulation |
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Authors: | Fernand F Fagutao Motoshige Yasuike Christopher Marlowe Caipang Hidehiro Kondo Ikuo Hirono Yukinori Takahashi Takashi Aoki |
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Institution: | (1) Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato-ku, Tokyo 184-8477, Japan;(2) Department of Applied Aquaculture, National Fisheries University, 2-7-1, Nagatahonmachi, Shimonoseki, Yamaguchi, Japan |
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Abstract: | Shrimps are believed to lack an adaptive immune system and therefore rely heavily on their innate immune mechanisms to ward
off pathogens. Moreover, their innate defense reactions are triggered by bacterial and fungal cell wall components such as
lipopolysaccharides, peptidoglycan and β-glucans. In this study, we used microarray to examine the gene expression profile
of kuruma shrimp, Marsupenaeus japonicus, after stimulation with peptidoglycan. Subsequent results show that the number of upregulated genes and percentage of differential
expression (21%) was highest at day 1 poststimulation. Differentially expressed genes in day 7 and day 14, on the other hand,
were 3.25% and 11.21%, respectively. Sixty-one (61) genes of unknown function were found to have responded outright to peptidoglycan
(PG) stimulation. Administration of PG also caused increases in the expressions of crustin, lysozyme, and a few antibacterial
peptides, all of which are known to be involved in crustacean immune response. Taken together, our results suggest that innate
response in shrimp is triggered instantaneously upon exposure to a bacterial component.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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Keywords: | Microarray Peptidoglycan Kuruma shrimp Innate immunity |
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